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Autor: Thomas H. Sharp
ISBN-13: 9783319021584
Einband: Buch
Seiten: 150
Gewicht: 394 g
Format: 244x163x17 mm
Sprache: Englisch

Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo

Springer Theses
With concise coverage of light- and electron-based microscopy, including new cryoelectron techniques, this readily consultable guide allows researchers to make informed decisions on which technique to use, and shows them how to interpret the resulting imagery.
Introduction.- Biomolecules & Microscopy.- Materials and Methods.-Experimental Methods.- Imaging Methods.- Experimental Results.- Towards an Atomistic Structure of a De Novo Designed Peptide Fibre.- Developing Novel Probes for Correlative Light Electron Microscopy.- Conclusions and Appendices.- Concluding Remarks.- Abbreviations.- Amino Acid Structures.- Vectors, Primers and Recipes.
As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.
Autor: Thomas H. Sharp
ISBN-13:: 9783319021584
ISBN: 3319021583
Verlag: Springer, Berlin, Springer International Publishing
Gewicht: 394g
Seiten: 150
Sprache: Englisch
Auflage 2014
Sonstiges: Buch, 244x163x17 mm, 30 SW-Abb., 55 Farbabb.